mouse anti-serca2 iid8 Search Results


serca2 atpase antibody (iid8) - bsa free  (Bio-Techne corporation)
94
Bio-Techne corporation serca2 atpase antibody (iid8) - bsa free
Serca2 Atpase Antibody (Iid8) Bsa Free, supplied by Bio-Techne corporation, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mouse+anti-serca2+iid8/SERCA2+ATPase+Antibody+(IID8)+-+BSA+Free/custom%40nb300-529%4040069352
Average 94 stars, based on 1 article reviews
serca2 atpase antibody (iid8) - bsa free - by Bioz Stars, 2026-09
94/100 stars
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mouse anti serca2 iid8 monoclonal antibody  (Santa Cruz Biotechnology)
94
Santa Cruz Biotechnology mouse anti serca2 iid8 monoclonal antibody
(A) 293T cells were transfected with a plasmid containing kGPCR. kGPCR was purified with anti-Flag affinity chromatography. kGPCR-interacting proteins were analyzed by commassie staining and photographed, identified by mass spectrometry analysis. A separating line indicates a lane that was removed between lanes of vector and kGPCR. Note, heat-denatured kGPCR oligomerized and migrated slowly. (B) 293T cells were transfected with plasmids containing <t>SERCA2</t> or kGPCR. Centrifuged cell extract was precipitated with anti-HA (kGPCR). Precipitated proteins and whole cell lysates were analyzed by immunoblot with indicated antibodies. (C and D) HUVEC endothelial stable cells were analyzed by proximity ligation assay (red) and stained with DAPI. Cells were analyzed by confocal microscopy (C). The intensity of proximity ligation was semi-quantitatively determined by counting more than 100 cells (D). (E) 293T cells were transfected with a plasmid containing Flag-tagged SERCA2 without or with a plasmid containing kGPCR. SERCA2 was precipitated with anti-Flag agarose and analyzed by ATPase activity in vitro. Precipitated SERCA2 and whole cell lysates were analyzed by immunoblot (insert). (F) 293T cells were transfected with an NFAT reporter cocktail, a plasmid containing kGPCR and increasing amount of a plasmid containing SERCA2. NFAT activation was determined by luciferase reporter assays.
Mouse Anti Serca2 Iid8 Monoclonal Antibody, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mouse+anti-serca2+iid8/SERCA2+Antibody/pmc04374719-235-30-35
Average 94 stars, based on 1 article reviews
mouse anti serca2 iid8 monoclonal antibody - by Bioz Stars, 2026-09
94/100 stars
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anti serca2 atpase  (Novus Biologicals)
93
Novus Biologicals anti serca2 atpase
(A) 293T cells were transfected with a plasmid containing kGPCR. kGPCR was purified with anti-Flag affinity chromatography. kGPCR-interacting proteins were analyzed by commassie staining and photographed, identified by mass spectrometry analysis. A separating line indicates a lane that was removed between lanes of vector and kGPCR. Note, heat-denatured kGPCR oligomerized and migrated slowly. (B) 293T cells were transfected with plasmids containing <t>SERCA2</t> or kGPCR. Centrifuged cell extract was precipitated with anti-HA (kGPCR). Precipitated proteins and whole cell lysates were analyzed by immunoblot with indicated antibodies. (C and D) HUVEC endothelial stable cells were analyzed by proximity ligation assay (red) and stained with DAPI. Cells were analyzed by confocal microscopy (C). The intensity of proximity ligation was semi-quantitatively determined by counting more than 100 cells (D). (E) 293T cells were transfected with a plasmid containing Flag-tagged SERCA2 without or with a plasmid containing kGPCR. SERCA2 was precipitated with anti-Flag agarose and analyzed by ATPase activity in vitro. Precipitated SERCA2 and whole cell lysates were analyzed by immunoblot (insert). (F) 293T cells were transfected with an NFAT reporter cocktail, a plasmid containing kGPCR and increasing amount of a plasmid containing SERCA2. NFAT activation was determined by luciferase reporter assays.
Anti Serca2 Atpase, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mouse+anti-serca2+iid8/SERCA2+ATPase+Antibody+(IID8)+%5BAllophycocyanin%5D/pmc11897295-90-131-140
Average 93 stars, based on 1 article reviews
anti serca2 atpase - by Bioz Stars, 2026-09
93/100 stars
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hif-1 alpha antibody - bsa free  (Bio-Techne corporation)
96
Bio-Techne corporation hif-1 alpha antibody - bsa free
(A) 293T cells were transfected with a plasmid containing kGPCR. kGPCR was purified with anti-Flag affinity chromatography. kGPCR-interacting proteins were analyzed by commassie staining and photographed, identified by mass spectrometry analysis. A separating line indicates a lane that was removed between lanes of vector and kGPCR. Note, heat-denatured kGPCR oligomerized and migrated slowly. (B) 293T cells were transfected with plasmids containing <t>SERCA2</t> or kGPCR. Centrifuged cell extract was precipitated with anti-HA (kGPCR). Precipitated proteins and whole cell lysates were analyzed by immunoblot with indicated antibodies. (C and D) HUVEC endothelial stable cells were analyzed by proximity ligation assay (red) and stained with DAPI. Cells were analyzed by confocal microscopy (C). The intensity of proximity ligation was semi-quantitatively determined by counting more than 100 cells (D). (E) 293T cells were transfected with a plasmid containing Flag-tagged SERCA2 without or with a plasmid containing kGPCR. SERCA2 was precipitated with anti-Flag agarose and analyzed by ATPase activity in vitro. Precipitated SERCA2 and whole cell lysates were analyzed by immunoblot (insert). (F) 293T cells were transfected with an NFAT reporter cocktail, a plasmid containing kGPCR and increasing amount of a plasmid containing SERCA2. NFAT activation was determined by luciferase reporter assays.
Hif 1 Alpha Antibody Bsa Free, supplied by Bio-Techne corporation, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mouse+anti-serca2+iid8/HIF-1+alpha+Antibody+-+BSA+Free/bio-techne+corporation___nb100-479
Average 96 stars, based on 1 article reviews
hif-1 alpha antibody - bsa free - by Bioz Stars, 2026-09
96/100 stars
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alpha fetoprotein / afp antibody  (NSJ Bioreagents)
99
NSJ Bioreagents alpha fetoprotein / afp antibody
(A) 293T cells were transfected with a plasmid containing kGPCR. kGPCR was purified with anti-Flag affinity chromatography. kGPCR-interacting proteins were analyzed by commassie staining and photographed, identified by mass spectrometry analysis. A separating line indicates a lane that was removed between lanes of vector and kGPCR. Note, heat-denatured kGPCR oligomerized and migrated slowly. (B) 293T cells were transfected with plasmids containing <t>SERCA2</t> or kGPCR. Centrifuged cell extract was precipitated with anti-HA (kGPCR). Precipitated proteins and whole cell lysates were analyzed by immunoblot with indicated antibodies. (C and D) HUVEC endothelial stable cells were analyzed by proximity ligation assay (red) and stained with DAPI. Cells were analyzed by confocal microscopy (C). The intensity of proximity ligation was semi-quantitatively determined by counting more than 100 cells (D). (E) 293T cells were transfected with a plasmid containing Flag-tagged SERCA2 without or with a plasmid containing kGPCR. SERCA2 was precipitated with anti-Flag agarose and analyzed by ATPase activity in vitro. Precipitated SERCA2 and whole cell lysates were analyzed by immunoblot (insert). (F) 293T cells were transfected with an NFAT reporter cocktail, a plasmid containing kGPCR and increasing amount of a plasmid containing SERCA2. NFAT activation was determined by luciferase reporter assays.
Alpha Fetoprotein / Afp Antibody, supplied by NSJ Bioreagents, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mouse+anti-serca2+iid8/Alpha+Fetoprotein+%2F+AFP+Antibody/custom%40v2002%4010%2E1161%2Fcircresaha%2E107%2E153676
Average 99 stars, based on 1 article reviews
alpha fetoprotein / afp antibody - by Bioz Stars, 2026-09
99/100 stars
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anti-rinsp3 t2  (Covalab Inc)
90
Covalab Inc anti-rinsp3 t2
(A) 293T cells were transfected with a plasmid containing kGPCR. kGPCR was purified with anti-Flag affinity chromatography. kGPCR-interacting proteins were analyzed by commassie staining and photographed, identified by mass spectrometry analysis. A separating line indicates a lane that was removed between lanes of vector and kGPCR. Note, heat-denatured kGPCR oligomerized and migrated slowly. (B) 293T cells were transfected with plasmids containing <t>SERCA2</t> or kGPCR. Centrifuged cell extract was precipitated with anti-HA (kGPCR). Precipitated proteins and whole cell lysates were analyzed by immunoblot with indicated antibodies. (C and D) HUVEC endothelial stable cells were analyzed by proximity ligation assay (red) and stained with DAPI. Cells were analyzed by confocal microscopy (C). The intensity of proximity ligation was semi-quantitatively determined by counting more than 100 cells (D). (E) 293T cells were transfected with a plasmid containing Flag-tagged SERCA2 without or with a plasmid containing kGPCR. SERCA2 was precipitated with anti-Flag agarose and analyzed by ATPase activity in vitro. Precipitated SERCA2 and whole cell lysates were analyzed by immunoblot (insert). (F) 293T cells were transfected with an NFAT reporter cocktail, a plasmid containing kGPCR and increasing amount of a plasmid containing SERCA2. NFAT activation was determined by luciferase reporter assays.
Anti Rinsp3 T2, supplied by Covalab Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mouse+anti-serca2+iid8/anti+rinsp3+t2/pmc03762358-140-25-30
Average 90 stars, based on 1 article reviews
anti-rinsp3 t2 - by Bioz Stars, 2026-09
90/100 stars
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vglut1 antibody  (Merck KGaA)
90
Merck KGaA vglut1 antibody
(A) 293T cells were transfected with a plasmid containing kGPCR. kGPCR was purified with anti-Flag affinity chromatography. kGPCR-interacting proteins were analyzed by commassie staining and photographed, identified by mass spectrometry analysis. A separating line indicates a lane that was removed between lanes of vector and kGPCR. Note, heat-denatured kGPCR oligomerized and migrated slowly. (B) 293T cells were transfected with plasmids containing <t>SERCA2</t> or kGPCR. Centrifuged cell extract was precipitated with anti-HA (kGPCR). Precipitated proteins and whole cell lysates were analyzed by immunoblot with indicated antibodies. (C and D) HUVEC endothelial stable cells were analyzed by proximity ligation assay (red) and stained with DAPI. Cells were analyzed by confocal microscopy (C). The intensity of proximity ligation was semi-quantitatively determined by counting more than 100 cells (D). (E) 293T cells were transfected with a plasmid containing Flag-tagged SERCA2 without or with a plasmid containing kGPCR. SERCA2 was precipitated with anti-Flag agarose and analyzed by ATPase activity in vitro. Precipitated SERCA2 and whole cell lysates were analyzed by immunoblot (insert). (F) 293T cells were transfected with an NFAT reporter cocktail, a plasmid containing kGPCR and increasing amount of a plasmid containing SERCA2. NFAT activation was determined by luciferase reporter assays.
Vglut1 Antibody, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mouse+anti-serca2+iid8/guinea+pig+anti+vglut1/pm31873081-259-50-52
Average 90 stars, based on 1 article reviews
vglut1 antibody - by Bioz Stars, 2026-09
90/100 stars
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goα antibody  (Merck KGaA)
90
Merck KGaA goα antibody
(A) 293T cells were transfected with a plasmid containing kGPCR. kGPCR was purified with anti-Flag affinity chromatography. kGPCR-interacting proteins were analyzed by commassie staining and photographed, identified by mass spectrometry analysis. A separating line indicates a lane that was removed between lanes of vector and kGPCR. Note, heat-denatured kGPCR oligomerized and migrated slowly. (B) 293T cells were transfected with plasmids containing <t>SERCA2</t> or kGPCR. Centrifuged cell extract was precipitated with anti-HA (kGPCR). Precipitated proteins and whole cell lysates were analyzed by immunoblot with indicated antibodies. (C and D) HUVEC endothelial stable cells were analyzed by proximity ligation assay (red) and stained with DAPI. Cells were analyzed by confocal microscopy (C). The intensity of proximity ligation was semi-quantitatively determined by counting more than 100 cells (D). (E) 293T cells were transfected with a plasmid containing Flag-tagged SERCA2 without or with a plasmid containing kGPCR. SERCA2 was precipitated with anti-Flag agarose and analyzed by ATPase activity in vitro. Precipitated SERCA2 and whole cell lysates were analyzed by immunoblot (insert). (F) 293T cells were transfected with an NFAT reporter cocktail, a plasmid containing kGPCR and increasing amount of a plasmid containing SERCA2. NFAT activation was determined by luciferase reporter assays.
Goα Antibody, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mouse+anti-serca2+iid8/anti+%CE%B1sma+mouse+monoclonal+antibody/pm31873081-259-44-46
Average 90 stars, based on 1 article reviews
goα antibody - by Bioz Stars, 2026-09
90/100 stars
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mouse monoclonal anti-human serca2 antibody clone iid8  (Biomol GmbH)
90
Biomol GmbH mouse monoclonal anti-human serca2 antibody clone iid8
(A) 293T cells were transfected with a plasmid containing kGPCR. kGPCR was purified with anti-Flag affinity chromatography. kGPCR-interacting proteins were analyzed by commassie staining and photographed, identified by mass spectrometry analysis. A separating line indicates a lane that was removed between lanes of vector and kGPCR. Note, heat-denatured kGPCR oligomerized and migrated slowly. (B) 293T cells were transfected with plasmids containing <t>SERCA2</t> or kGPCR. Centrifuged cell extract was precipitated with anti-HA (kGPCR). Precipitated proteins and whole cell lysates were analyzed by immunoblot with indicated antibodies. (C and D) HUVEC endothelial stable cells were analyzed by proximity ligation assay (red) and stained with DAPI. Cells were analyzed by confocal microscopy (C). The intensity of proximity ligation was semi-quantitatively determined by counting more than 100 cells (D). (E) 293T cells were transfected with a plasmid containing Flag-tagged SERCA2 without or with a plasmid containing kGPCR. SERCA2 was precipitated with anti-Flag agarose and analyzed by ATPase activity in vitro. Precipitated SERCA2 and whole cell lysates were analyzed by immunoblot (insert). (F) 293T cells were transfected with an NFAT reporter cocktail, a plasmid containing kGPCR and increasing amount of a plasmid containing SERCA2. NFAT activation was determined by luciferase reporter assays.
Mouse Monoclonal Anti Human Serca2 Antibody Clone Iid8, supplied by Biomol GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mouse+anti-serca2+iid8/serca1+antibody/pm16467572-235-34-35
Average 90 stars, based on 1 article reviews
mouse monoclonal anti-human serca2 antibody clone iid8 - by Bioz Stars, 2026-09
90/100 stars
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cytochrome c antibody  (NSJ Bioreagents)
99
NSJ Bioreagents cytochrome c antibody
(A) 293T cells were transfected with a plasmid containing kGPCR. kGPCR was purified with anti-Flag affinity chromatography. kGPCR-interacting proteins were analyzed by commassie staining and photographed, identified by mass spectrometry analysis. A separating line indicates a lane that was removed between lanes of vector and kGPCR. Note, heat-denatured kGPCR oligomerized and migrated slowly. (B) 293T cells were transfected with plasmids containing <t>SERCA2</t> or kGPCR. Centrifuged cell extract was precipitated with anti-HA (kGPCR). Precipitated proteins and whole cell lysates were analyzed by immunoblot with indicated antibodies. (C and D) HUVEC endothelial stable cells were analyzed by proximity ligation assay (red) and stained with DAPI. Cells were analyzed by confocal microscopy (C). The intensity of proximity ligation was semi-quantitatively determined by counting more than 100 cells (D). (E) 293T cells were transfected with a plasmid containing Flag-tagged SERCA2 without or with a plasmid containing kGPCR. SERCA2 was precipitated with anti-Flag agarose and analyzed by ATPase activity in vitro. Precipitated SERCA2 and whole cell lysates were analyzed by immunoblot (insert). (F) 293T cells were transfected with an NFAT reporter cocktail, a plasmid containing kGPCR and increasing amount of a plasmid containing SERCA2. NFAT activation was determined by luciferase reporter assays.
Cytochrome C Antibody, supplied by NSJ Bioreagents, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mouse+anti-serca2+iid8/Cytochrome+C+Antibody/custom%40rq4499%4016467572
Average 99 stars, based on 1 article reviews
cytochrome c antibody - by Bioz Stars, 2026-09
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mouse anti human akt  (Cell Signaling Technology Inc)
96
Cell Signaling Technology Inc mouse anti human akt
(A) 293T cells were transfected with a plasmid containing kGPCR. kGPCR was purified with anti-Flag affinity chromatography. kGPCR-interacting proteins were analyzed by commassie staining and photographed, identified by mass spectrometry analysis. A separating line indicates a lane that was removed between lanes of vector and kGPCR. Note, heat-denatured kGPCR oligomerized and migrated slowly. (B) 293T cells were transfected with plasmids containing <t>SERCA2</t> or kGPCR. Centrifuged cell extract was precipitated with anti-HA (kGPCR). Precipitated proteins and whole cell lysates were analyzed by immunoblot with indicated antibodies. (C and D) HUVEC endothelial stable cells were analyzed by proximity ligation assay (red) and stained with DAPI. Cells were analyzed by confocal microscopy (C). The intensity of proximity ligation was semi-quantitatively determined by counting more than 100 cells (D). (E) 293T cells were transfected with a plasmid containing Flag-tagged SERCA2 without or with a plasmid containing kGPCR. SERCA2 was precipitated with anti-Flag agarose and analyzed by ATPase activity in vitro. Precipitated SERCA2 and whole cell lysates were analyzed by immunoblot (insert). (F) 293T cells were transfected with an NFAT reporter cocktail, a plasmid containing kGPCR and increasing amount of a plasmid containing SERCA2. NFAT activation was determined by luciferase reporter assays.
Mouse Anti Human Akt, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mouse+anti-serca2+iid8/Akt+(pan)+Mouse+mAb/pmc06459310-322-41-45
Average 96 stars, based on 1 article reviews
mouse anti human akt - by Bioz Stars, 2026-09
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(A) 293T cells were transfected with a plasmid containing kGPCR. kGPCR was purified with anti-Flag affinity chromatography. kGPCR-interacting proteins were analyzed by commassie staining and photographed, identified by mass spectrometry analysis. A separating line indicates a lane that was removed between lanes of vector and kGPCR. Note, heat-denatured kGPCR oligomerized and migrated slowly. (B) 293T cells were transfected with plasmids containing SERCA2 or kGPCR. Centrifuged cell extract was precipitated with anti-HA (kGPCR). Precipitated proteins and whole cell lysates were analyzed by immunoblot with indicated antibodies. (C and D) HUVEC endothelial stable cells were analyzed by proximity ligation assay (red) and stained with DAPI. Cells were analyzed by confocal microscopy (C). The intensity of proximity ligation was semi-quantitatively determined by counting more than 100 cells (D). (E) 293T cells were transfected with a plasmid containing Flag-tagged SERCA2 without or with a plasmid containing kGPCR. SERCA2 was precipitated with anti-Flag agarose and analyzed by ATPase activity in vitro. Precipitated SERCA2 and whole cell lysates were analyzed by immunoblot (insert). (F) 293T cells were transfected with an NFAT reporter cocktail, a plasmid containing kGPCR and increasing amount of a plasmid containing SERCA2. NFAT activation was determined by luciferase reporter assays.

Journal: PLoS Pathogens

Article Title: Herpesviral G Protein-Coupled Receptors Activate NFAT to Induce Tumor Formation via Inhibiting the SERCA Calcium ATPase

doi: 10.1371/journal.ppat.1004768

Figure Lengend Snippet: (A) 293T cells were transfected with a plasmid containing kGPCR. kGPCR was purified with anti-Flag affinity chromatography. kGPCR-interacting proteins were analyzed by commassie staining and photographed, identified by mass spectrometry analysis. A separating line indicates a lane that was removed between lanes of vector and kGPCR. Note, heat-denatured kGPCR oligomerized and migrated slowly. (B) 293T cells were transfected with plasmids containing SERCA2 or kGPCR. Centrifuged cell extract was precipitated with anti-HA (kGPCR). Precipitated proteins and whole cell lysates were analyzed by immunoblot with indicated antibodies. (C and D) HUVEC endothelial stable cells were analyzed by proximity ligation assay (red) and stained with DAPI. Cells were analyzed by confocal microscopy (C). The intensity of proximity ligation was semi-quantitatively determined by counting more than 100 cells (D). (E) 293T cells were transfected with a plasmid containing Flag-tagged SERCA2 without or with a plasmid containing kGPCR. SERCA2 was precipitated with anti-Flag agarose and analyzed by ATPase activity in vitro. Precipitated SERCA2 and whole cell lysates were analyzed by immunoblot (insert). (F) 293T cells were transfected with an NFAT reporter cocktail, a plasmid containing kGPCR and increasing amount of a plasmid containing SERCA2. NFAT activation was determined by luciferase reporter assays.

Article Snippet: Commercial antibodies used in this study include mouse anti-HA monoclonal antibody and agarose (Sigma), mouse anti- β-Actin monoclonal antibody (Abcam), rabbit anti-COX-2 polyclonal antibody (Abcam), rabbit anti-RCAN1 polyclonal antibody (Sigma), mouse anti-SERCA2 (IID8) monoclonal antibody (Santa cruz), mouse anti-calcineurin Aα (Santa cruz).

Techniques: Transfection, Plasmid Preparation, Purification, Affinity Chromatography, Staining, Mass Spectrometry, Western Blot, Proximity Ligation Assay, Confocal Microscopy, Ligation, Activity Assay, In Vitro, Activation Assay, Luciferase